Method of producing 2,3-butanediol with high yield

A method is provided for producing 2,3-butanediol. An escherichia coli (E. coli) strain obtained through an innovation of genetic modification is used. In vivo of the strain, desired genes for producing 2,3-butanediol are expressed through gene transfer in a gene-recombination way. The present inven...

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Bibliographic Details
Main Authors MA, TIEN-YANG, LIANG, KE-MING, SHEN, ROA-PU, GUO, GIA-LUEN, HUANG, CHIUNG-FANG
Format Patent
LanguageChinese
English
Published 16.10.2019
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A method is provided for producing 2,3-butanediol. An escherichia coli (E. coli) strain obtained through an innovation of genetic modification is used. In vivo of the strain, desired genes for producing 2,3-butanediol are expressed through gene transfer in a gene-recombination way. The present invention is characterized in that, during producing 2,3-butanediol, the strain does not change its yield owing to the change of oxygen concentration in the fermentation environment. The strain is capable of self-regulation by using an in-vivo metabolic pathway for dealing with the external environmental oxygen concentration. Thus, 2,3-butanediol is continuously produced with a high yield.
Bibliography:Application Number: TW20187109789