Strain capable of producing 1-butanol and applications thereof which belongs to Escherichia coli and lacks a pgi gene, an IdhA gene, a pta gene, an adhE gene, a frdA gene

• Main Authors: SAINI, MUKESH, ZHAO, YUN-PENG, JIANG, ZHONG-REN
• Format: Patent
• Language: Chinese; English
• Published: 01.03.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

A Strain capable of producing 1-butanol belongs to Escherichia coli and comprises: an hbd gene of clostridium acetobutylicum, a crt gene of clostridium acetobutylicum, an adhE2 gene, a phaA gene, a ter gene of treponema denticola, a fdh1 gene of saccharomyces cerevisiae, a lambda pl promoter (P [lambda]PL), a lac operon (lacO). The lambda pl promoter P[lambda]PL is a chromosome inlaid in the strain to control a zwf gene, a pgl gene, an udhA gene and an aceEF gene operon-mutated lpdA gene (E354K) on the chromosome. The lac operon lacO is a chromosome inlaid in the strain to control a glta gene on the chromosome. The strain lacks a pgi gene, an IdhA gene, a pta gene, an adhE gene, a frdA gene.