Molecular probe chip with covalent bonding anchoring compound

• Main Authors: LIN, CHII WANN, LEE, SHIH-YUAN, LIN, PANIEN, CHEN, JI-LIANG, LIN, SHIMING, HSU, SU MING, LEE, CHIH-KUANG, LIN, YUN HAN
• Format: Patent
• Language: Chinese; English
• Published: 16.10.2005
• Subjects: ACYCLIC OR CARBOCYCLIC COMPOUNDS; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

A molecular probe chip is disclosed having covalent bonding anchoring compounds formed on the chip substrate in a chemical modification. The covalent bonding anchoring compounds allow for fast and precision molecular sample testing, examination and characterization via covalent bonding with corresponding functional group of a sample. Covalent bonding of the probes with the anchoring compounds of the chip substrate improves the stability of the molecular probe chip. Due to such stability, a test chip can be washed with adequate solution without substantial lost of molecular compound already bonded to the probes. Thorough cleaning of the chip reduces to minimum the interference from debris to the test, which leads to improved precision in the examination and characterization results. A molecular probe chip comprises a substrate having coated to a surface thereof a layer of metallic film. First end of each of a number of elongated anchoring compounds being bounded to the metallic film of the substrate in a coval