PROCESS FOR PRODUCING L-TREONINE

• Main Authors: LIVSHITS VITALIJ A,SU, KOZLOV YURIJ I,SU, KHURGES EVGENIJ M,SU, DEBABOV VLADIMIR G,SU, SOKOLOV ALEKSANDR K,SU, ZHDANOVA NELLI I,SU, YANKOVSKIJ NIKOLAJ K,SU, GUSYATINER MIKHAIL M,SU, SHOLIN ALBERT F,SU, ANTIPOV VIKTOR P,SU, POZDNYAKOVA TAMARA M,SU
• Format: Patent
• Language: English
• Published: 15.07.1982
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; VINEGAR; WINE

The process for producing L-threonine consists in that there is cultivated a producer of L-threonine in the capacity of which is used the Escherichia coli strain VNIIgenetika M-1 deposited in the Central museum of commercial microorganisms under the All-Union Research Institute for Genetics and Selection of Commercial Microorganisms at a registration No. IIMIIB-1856. The above strain has been selected on the basis of natural variability of the Escherichia coli strain VNIIgenetika VL 334/p YN7), obtained by virtue of the genetic engineering techniques through increasing the dose of mutant genes capable of a higher rate of L-threonine production, by introducing a multicopy hydrid plasmid carrying said genes, into a mutant recipient strain. The abovesaid producer is cultivated on a nutrient medium, containing sources of carbon, nitrogen, and some mineral salts in the presence of an antibiotic penicillin, the resultant biomass being then separated from the culture fluid, whereupon the end product is isolated.