METHOD FOR PURIFICATION OF RECOMBINATE PROTEIN WHICH CONTAINS PROINSULIN SEQUENCE

FIELD: biotechnology. SUBSTANCE: method is carried out by chromatographic purification on anionite. Chromatography takes place on copolymer of ethylene glycol dimethylacrylate with 2-hydroxy-3-diethylaminopropyl methacrylate, 0.007-0.013 M acetic acid which contains 0.15-0.30 M of sodium acetate bei...

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Main Authors Gromova O.A, Korshunov M.A, Beljaev S.V, Khrushcheva T.A, Melekhov V.M, Gavrjuchenkova L.P, Kulikov S.V, Tsukanova L.N, Mikhlin V.S, Mal'tsev K.V, Leonova E.B, Vul'fson A.N, Morozov S.M
Format Patent
LanguageEnglish
Russian
Published 27.04.1996
Subjects
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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Summary:FIELD: biotechnology. SUBSTANCE: method is carried out by chromatographic purification on anionite. Chromatography takes place on copolymer of ethylene glycol dimethylacrylate with 2-hydroxy-3-diethylaminopropyl methacrylate, 0.007-0.013 M acetic acid which contains 0.15-0.30 M of sodium acetate being used. Protein is eluted by 0.8-1.1 M acetic acid. Yield of protein is up to 73 %. EFFECT: increases yield of desired product. 2 tbl Использование: биотехнология. Сущность изобретения: хроматографическая очистка на анионите. С целью повышения выхода целевого продукта хроматографию проводят на сополимере диметакрилата этиленгликоля с 2-гидрокси-3-диэтиламинопропилметакрилатом в 0,007 - 0,013 М уксусной кислоте, содержащей 0,15 - 0,30 М ацетата натрия, а белок элюируют 0,8 - 1,1 М уксусной кислотой. Выход белка до 73%. 2 табл.
Bibliography:Application Number: SU19904838187