METHOD FOR PREPARATION OF COMPLEX PLATINUM (II) COMPOUND WITH HIGH-MOLECULAR N-DNA FROM SPLEEN OF CATTLE, GRADE A, SHOWING ANTICARCINOGENIC ACTIVITY

• Main Authors: BUDARIN LEV I,SU, TROKHIMENKO ELENA P,SU, KEJSEVICH LYUDVIG V,SU, SHALIMOV SERGEJ A,SU, MAJDANEVICH NADEZHDA N,SU, VOLCHENSKOVA ILIMA I,SU
• Format: Patent
• Language: English; Russian
• Published: 23.10.1991
• Subjects: ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAININGELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN,SULFUR, SELENIUM OR TELLURIUM; CHEMISTRY; DERIVATIVES THEREOF; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SUGARS

Derivatives of platinum (P) with polyanion of deoxyribonucleic acid have the following general formula (I): {[PtCl(NH3)]6(OH)x(H2O)y}n where DNA<-m> = ( C39H55-rO32N15P4 )n<-m>; n = 2000+/-200; when x = 0, y = 12, m = 6n, r = 6; when x = 2, y = 10, m = 4n, r = 4; when x = 0, y = 6, m = 6n, r = 6. A method of obtaining said combinations consists in reacting deoxyribonucleic acid having a molecular mass of at least 8.10 D with cis-dichloramine-platinum (P) or with partially hydrolyzed cis-dichloramine-platinum (P) in the aqueous medium or in a buffer solution with a pH of 7.0 to 7.5 and with the ion power of 0.01 to 0.02 at a temperature not less than the melting point of the deoxyribonucleic acid with subsequent extraction of the desired product. The claimed combinations have an antitumor activity. A pharmaceutical preparation of antitumor action comprises as an active component the claimed combination: poly{hexakis[chloraminaquaplatinum(P)]}- mu -deoxyribonucleate.