HUMAN IMMUNODEFICIENCY VIRUS (HIV)-NEUTRALIZING ANTIBODIES

• Main Authors: Chan-Hui Po-ying, Burton Dennis, Moyle Matthew, Frey Steven, Phogat Sanjay K, de Jean de St. Marcel Simek-Lemos Melissa D, Walker Laura Marjorie, Kaminsky Stephen, Mitcham Jennifer, Olsen Ole, Koff Wayne
• Format: Patent
• Language: English; Slovenian
• Published: 30.07.2021
• Subjects: CHEMISTRY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

The invention provides a method for obtaining a broadly neutralizing antibody (bNab), wherein said antibody is PGG14 having a heavy chain comprising the amino acid sequence of SEQ ID NO: 20 and a light chain comprising the amino acid sequence of SEQ ID NO: 22. Said method includes screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibody of the invention is characterized by its ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing said human monoclonal anti-HIV antibody of the invention, which are used for prophylaxis, diagnosis and treatment of HIV infection, are provided. Methods for generating such antibody by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIVl bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided.