COMPOSITIONS FOR REPROGRAMMING CELLS INTO DENDRITIC CELLS OR ANTIGEN PRESENTING CELLS, METHODS AND USES THEREOF

• Main Authors: FIÚZA ROSA, Fábio Alexandre, RIBEIRO LEMOS PEREIRA, Carlos Filipe, FERREIRA PIRES, Cristiana
• Format: Patent
• Language: English
• Published: 30.05.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Publishedwith international search report (Art. (3)) with sequence listing part of description (Rule 5.2(a)) in black and white; the international application as filed contained color or greyscale and is available for download from PATENTSCOPE (25) Filing Language: (26) Publication Language: (30) Priority Data: 110012 17171166.6 110263 110267 05 April 2018 (05.04.2018) English English 05 April 2017 (05.04.2017) PT 15 May 2017 (15.05.2017) EP 24 August 2017 (24.08.2017) PT 25 August 2017 (25.08.2017) PT O N 00 00 O O (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 11 October 2018 (11.10.2018) WIPO I PCT onion °nolo HE ioo mo Homo iflo oimIE (10) International Publication Number WO 2018/185709 Al (51) International Patent Classification: C12N 5/0784 (2010.01) A61K 35/15 (2015.01) (21) International Application Number: PCT/IB2018/052378 (22) International Filing Date: TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (71) Applicant: CENTRO DE NEUROCIENCIAS E BIOLOGIA CELULAR [PT/PT]; Universidade de Coim- bra;, Center for Neuroscience and Cell Biology (CNC);, Largo Marques do Pombal, 3004-517 Coimbra (PT). (72) Inventors: RIBEIRO LEMOS PEREIRA, Carlos Filipe; Rua Joao Baptista da Silva 390, Moledo, 4910-264 Cam- inha (PT). FERREIRA PIRES, Cristiana; Rua Fonte do Areeiro n29, 3770-352 Palhaca (PT). FIUZA ROSA, Fabio Alexandre; Rua do Brejo, N°53 Touria Pousos, 2410-205 Leiria (PT). (74) Agent: TEIXEIRA DE CARVALHO, Anabela; Paten- tree, Edificio Net, Rua de Salazares 842, 4149-002 Porto (PT). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, (54) Title: COMPOSITIONS FOR REPROGRAMMING CELLS INTO DENDRITIC CELLS OR ANTIGEN PRESENTING CELLS, METHODS AND USES THEREOF (57) : The present disclosure relates to compositions, nucleic acid constructs, methods and kits thereof for cell induction or reprogramming cells to the dendritic cell state or antigen presenting cell state, based, in part, on the surprisingly effect described herein of novel use and combinations of transcription factors that permit induction or reprogramming of differentiated or undifferentiated cells into dendritic cells or antigen presenting cells. Such compositions, nucleic acid constructs, methods and kits can be used for inducing dendritic cells in vitro, ex vivo, or in vivo, and these induced dendritic cells or antigen presenting cells can be used for immunotherapy applications.