METHOD FOR PRODUCING HIGH-QUALITY RECOMBINANT ALLERGENS IN A PLANT

A method for producing a recombinant protein in a plant, in particular a tobacco plant, preferably Nicotiana benthamiana, includes the following steps: a) culturing the plant aeroponically or hydroponically, preferably on mobile floats and under LED lighting; b) vacuum agroinfiltration of the plant...

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Bibliographic Details
Main Authors GOMORD, VÉRONIQUE, FAYE, LOÏC, FITCHETTE, ANNE CATHERINE
Format Patent
LanguageEnglish
Published 29.01.2015
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A method for producing a recombinant protein in a plant, in particular a tobacco plant, preferably Nicotiana benthamiana, includes the following steps: a) culturing the plant aeroponically or hydroponically, preferably on mobile floats and under LED lighting; b) vacuum agroinfiltration of the plant obtained in a) by agrobacteria that include a DNA fragment coding for the recombinant protein; c) returning the plants to culture after step b), under the same conditions as for step a); and d) extracting and purifying the recombinant protein from the aerial portions of the plants produced in step c).
Bibliography:Application Number: SG20141108214W