METHOD FOR DETECTING DNA OF TORQUE TENO VIRUS OF GENUS ALPHATORQUEVIRUS FAMILY OF ANELLOVIRIDAE IN BLOOD AND OTHER BIOMATERIALS BY RT-PCR

• Main Authors: Lolomadze Elena Anatolevna, Rebrikov Denis Vladimirovich
• Format: Patent
• Language: English; Russian
• Published: 26.02.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

FIELD: biotechnology.SUBSTANCE: invention relates to the field of biotechnology. Disclosed are synthetic oligonucleotides for detecting DNA of Torque teno virus of genus Alphatorquevirus family Anelloviridae. Primers are combined into kits for detecting DNA in blood and other biological materials of Torque teno virus of genus Alphatorquevirus family Anelloviridae by polymerase chain reaction. Invention enables reliable detection of said virus in a biological material.EFFECT: using this method provides also highly sensitive and objective quantitative assessment of Torque teno virus.1 cl, 2 tbl Изобретение относится к области биотехнологии. Раскрыты синтетические олигонуклеотиды для выявления ДНК вируса Torque teno virus рода Alphatorquevirus семейства Anelloviridae. Праймеры объединены в наборы для выявления ДНК в крови и других биологических материалах вируса Torque teno virus рода Alphatorquevirus семейства Anelloviridae методом полимеразной цепной реакции. Изобретение позволяет достоверно проводить обнаружение указанного вируса в биологическом материале. Использование данного метода обеспечивает также высокочувствительную и объективную количественную оценку вируса Torque teno virus. 2 табл.