RECOMBINANT DNA FRAGMENT, ENCODING HUMAN ALPHA-FETOPROTEIN (AFP), CONTAINING "SLOW" LEUCINE-ENCODING CODONS, EXPRESSION PLASMID, CONTAINING SAID FRAGMENT, SACCHAROMYCES CEREVISIAE CELL, TRANSFORMED WITH SAID PLASMID, AND METHOD FOR OBTAINING HUMAN AFP

FIELD: medicine.SUBSTANCE: invention relates to field of biotechnology, can be used for obtaining human alpha-fetoprotein (AFP) with application of strains of Saccharomyces cerevisiae yeasts, containing recombinant AFP gene. Obtained are: recombinant human AFT, containing so-called "slow"...

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Main Authors Dudich Igor Vyacheslavovich, Morozkina Elena Vladimirovna, Zatsepin Sergej Sergeevich, Ostroumov Yurij Igorevich, Semenkova Lidiya Nikolaevna, Vavilova Ekaterina Aleksandrovna, Benevolenskij Sergej Vladimirovich
Format Patent
LanguageEnglish
Russian
Published 28.04.2017
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:FIELD: medicine.SUBSTANCE: invention relates to field of biotechnology, can be used for obtaining human alpha-fetoprotein (AFP) with application of strains of Saccharomyces cerevisiae yeasts, containing recombinant AFP gene. Obtained are: recombinant human AFT, containing so-called "slow" codons, encoding leucine in conservative AFP sections, recombinant plasmid and Saccharomyces cerevisiae cell for expression and secretion of said recombinant AFP respectively.EFFECT: invention makes it possible to increase production of active AFP.9 cl, 16 dwg, 3 tbl, 6 ex Изобретение относится к области биотехнологии, может быть использовано для получения альфа-фетопротеина (АФП) человека с использованием штаммов дрожжей Saccharomyces cerevisiae, содержащих рекомбинантный ген АФП. Получают рекомбинантный АФП человека, содержащий так называемые "медленные" кодоны, кодирующие лейцин в консервативных участках АФП, рекомбинантную плазмиду и клетку Saccharomyces cerevisiae для экспрессии и секреции указанного рекомбинантного АФП соответственно. Изобретение позволяет повысить продукцию активного АФП. 4 н. и 5 з.п. ф-лы, 16 ил., 3 табл., 6 пр.
Bibliography:Application Number: RU20150129623