DETERMINATION OF LATENT ACTIVITY DYNAMIC FOR LEUCOCYTE HOMOGENATE LACTATE DEHYDROGENASE (LDH) ISOENZYMES

• Main Authors: SEMKE VALENTIN JAKOVLEVICH, EPANCHINTSEVA ELENA MAKAROVNA, TEROVSKIJ STANISLAV STANISLAVOVICH, KUSKOV MIKHAIL VIKTOROVICH, FEDORENKO OL'GA JUR'EVNA, IVANOVA SVETLANA ALEKSANDROVNA
• Format: Patent
• Language: English; Russian
• Published: 10.02.2007
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

FIELD: biochemistry. ^ SUBSTANCE: claimed method includes incubation step before electrophoresis separation of LDH isoenzymes. Cell homogenate is divided into 8 equal aliquots. Three aliquots are incubated for 15 min at 37°C with urea at concentration of 1, 2, 3 Mol; the next three aliquots are incubated with the same urea concentration in presence of Triton N-101 detergent at 1 % concentration thereof. Then difference between activity of corresponding LDH isoenzymes in gel after leucocyte homogenate incubation in presence of detergent and in absence thereof is calculated. One of the two rest aliquot is incubated for 15 min at 37°C without addition of reagent, and the other one is incubated under the same conditions in presence of Triton N-101 detergent at 1 % concentration. Then difference between activity of corresponding LDH isoenzymes in gel after leucocyte homogenate incubation without reagents and in presence of detergent. ^ EFFECT: new method for investigation of cell homogenate aggregate state in diagnosis of pathologies associated with cell homogenate alteration. ^ 3 tbl, 3 dwg