METHOD FOR PRESERVING SMALL DOSES OF ANTIGEN IN LIQUID NITROGEN

• Main Authors: POZOV S.A, TRUKHACHEV V.I, ORLOVA N.E
• Format: Patent
• Language: English; Russian
• Published: 20.09.2004
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

FIELD: microbiology, medicine. ^ SUBSTANCE: invention relates, in particular, to methods for preserving diagnostic preparations. Antigen drops of size 35-40 mm3 are dropped by Pasteur's pipette or syringe (volume is 2-3 ml) with needle on liquid nitrogen surface. In the process of freezing antigen drops form "globules" that fell on tube bottom. Freezing temperature under these conditions is (-170)-(-180)oC and time for formation of "globules" is 1 min. Then "globules" are transferred into plastic bottles in common with nitrogen for storing. Method provides retention of activity and specificity of antigen for a prolonged time. ^ EFFECT: improved preserving method. ^ 4 tbl, 3 ex