METHOD FOR PRESERVING SMALL DOSES OF ANTIGEN IN LIQUID NITROGEN

FIELD: microbiology, medicine. ^ SUBSTANCE: invention relates, in particular, to methods for preserving diagnostic preparations. Antigen drops of size 35-40 mm3 are dropped by Pasteur's pipette or syringe (volume is 2-3 ml) with needle on liquid nitrogen surface. In the process of freezing anti...

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Bibliographic Details
Main Authors POZOV S.A, TRUKHACHEV V.I, ORLOVA N.E
Format Patent
LanguageEnglish
Russian
Published 20.09.2004
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
HUMAN NECESSITIES
HYGIENE
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PHYSICS
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TESTING
VINEGAR
WINE
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Summary:FIELD: microbiology, medicine. ^ SUBSTANCE: invention relates, in particular, to methods for preserving diagnostic preparations. Antigen drops of size 35-40 mm3 are dropped by Pasteur's pipette or syringe (volume is 2-3 ml) with needle on liquid nitrogen surface. In the process of freezing antigen drops form "globules" that fell on tube bottom. Freezing temperature under these conditions is (-170)-(-180)oC and time for formation of "globules" is 1 min. Then "globules" are transferred into plastic bottles in common with nitrogen for storing. Method provides retention of activity and specificity of antigen for a prolonged time. ^ EFFECT: improved preserving method. ^ 4 tbl, 3 ex
Bibliography:Application Number: RU20020121543