METHOD FOR PREPARING PURIFIED PREPARATION OF BOTULINIC TOXIN TYPE A

FIELD: microbiology, toxicology. SUBSTANCE: invention relates to methods for purification of preparations of botulinic toxin. The strain-producer Clostridium botulinum is cultured under anaerobic conditions. Toxic-containing culture is centrifuged and toxin is extracted from precipitate prepared. Is...

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Bibliographic Details
Main Authors TELITSYN L.M, BAKULINA L.V, GRACHEV O.E, DUBROVIN M.JU, PJATKOV V.A, ELAGIN G.D, RUDOJ B.A
Format Patent
LanguageEnglish
Russian
Published 10.06.2004
Edition7
Subjects
HUMAN NECESSITIES
HYGIENE
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEDICAL OR VETERINARY SCIENCE
PHYSICS
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
TESTING
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Summary:FIELD: microbiology, toxicology. SUBSTANCE: invention relates to methods for purification of preparations of botulinic toxin. The strain-producer Clostridium botulinum is cultured under anaerobic conditions. Toxic-containing culture is centrifuged and toxin is extracted from precipitate prepared. Isolation of neurotoxin is carried out under conditions optimal for dissociation of components of toxic complex at pH 7.9 and in the concentration of sodium phosphate salt 0.025 M. Method provides reducing time for preparing neurotoxin, amount of stages in isolation of the preparation and to elevate the yield of the end product. EFFECT: improved preparing method. 2 tbl, 4 ex
Bibliography:Application Number: RU20020122179