METHOD FOR PREPARING DOXORUBICIN AND AGENTS FOR ITS REALIZATION

FIELD: genetic engineering, antibiotics. SUBSTANCE: as result of screening the genome library of the strain Streptomyces peucetius (ATCC 29050) DNA fragment is cloned that comprises gene encoding daunorubicin 14-hydroxylase. By transformation of strains Streptomyces and E. coli with vector containin...

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Bibliographic Details
Main Authors SKOTTI KLAUDIO, SOLARI INVENTI AVGUSTO, BREME UMBERTO, KOLOMBO ANNA LUIZA, KHATCHINSON CHARLZ RICHARD, OTTEN SHERI
Format Patent
LanguageEnglish
Russian
Published 27.05.2003
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:FIELD: genetic engineering, antibiotics. SUBSTANCE: as result of screening the genome library of the strain Streptomyces peucetius (ATCC 29050) DNA fragment is cloned that comprises gene encoding daunorubicin 14-hydroxylase. By transformation of strains Streptomyces and E. coli with vector containing the indicated gene recombinant strains exhibiting capacity to convert daunorubicin to doxorubicin effectively in culturing have been obtained. Invention can be used for industrial preparing antibiotic doxorubicin eliciting an antitumor activity. EFFECT: improved preparing method of doxorubicin. 6 cl, 3 dwg
Bibliography:Application Number: RU19960122866