METHOD OF ISOLATION OF PLAGUE PATHOGEN FROM ECTOPARASITES, FOR EXAMPLE, FLEAS

FIELD: medicinal microbiology. SUBSTANCE: method involves subcutaneous administration of ectoparasites suspension in physiological solution to experimental animals (0.5-1.0 ml in back region) and suspension is mixed with rabbit heparinized blood at ratio = (1:0.5)-(1:0.7). Then 0.5-0.7 ml of chicken...

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Main Authors TSAREVA N.S, EVCHENKO JU.M, GRIZHEBOVSKIJ G.M, SHCHEDRIN V.I, BRJUKHANOVA G.D, MEZENTSEV V.M, LOGVINENKO O.V
Format Patent
LanguageEnglish
Published 20.07.1999
Edition6
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
TESTING
VINEGAR
WINE
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Summary:FIELD: medicinal microbiology. SUBSTANCE: method involves subcutaneous administration of ectoparasites suspension in physiological solution to experimental animals (0.5-1.0 ml in back region) and suspension is mixed with rabbit heparinized blood at ratio = (1:0.5)-(1:0.7). Then 0.5-0.7 ml of chicken egg yolk, 5-7 mg of cortisone, 0.01-0.03 mg of potassium tellurate and 0.001-0.003 mg of gentian violet are added to the mixture. Bacteriological study of pathological material is carried out from the place of administration only being 0.02-0.03 mcg/ml of gentian violet is added to the solid nutrient medium. Invention can be used for bacteriological diagnosis of objects causing suspicion with respect to factors of plague infection transferring. EFFECT: improved method of isolation. 3 ex
Bibliography:Application Number: RU19980112323