METHOD OF HEPARIN ASSAY

FIELD: biotechnology, pharmacy, analytical biochemistry. SUBSTANCE: method involves formation of lyotropic mesomorphic dispersion from a complex of linear double-stranded DNA molecules of low molecular mass and heparin molecules as an antagonist and heparin is determined in an aqueous-salt solution....

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Bibliographic Details
Main Authors EVDOKIMOV JU.M, EFIMOV V.S, TEHRNER EHNTONI, SKURIDIN S.G, NEKRASOV A.V
Format Patent
LanguageEnglish
Russian
Published 10.12.1998
Edition6
Subjects
CHEMISTRY
DERIVATIVES THEREOF
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
PHYSICS
SUGARS
TESTING
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Summary:FIELD: biotechnology, pharmacy, analytical biochemistry. SUBSTANCE: method involves formation of lyotropic mesomorphic dispersion from a complex of linear double-stranded DNA molecules of low molecular mass and heparin molecules as an antagonist and heparin is determined in an aqueous-salt solution. The prepared dispersion is mixed firstly with a polymer an aqueous-saline solution that is neutral with respect to DNA, antagonist and heparin and then with heparin-containing liquid to be analyzed. Mixing is carried out at conditions where optical properties of lyotropic mesomorphic dispersion complex (DNA-antagonist) do not impaired. Then a flow of circularly polarized radiation is passed through a sample obtained by the indicated mixing and optical signal is recorded by wave-length in the range of DNA absorption. EFFECT: simplified and accelerated process of diagnosis of preclinical phase of pathological state. 4 cl, 1 tbl, 4 ex
Bibliography:Application Number: RU19970118583