RECOMBINANT PLASMID DNA ENCODING HUMAN PREPROINSULIN

FIELD: molecular biology, genetic engineering, medicine. SUBSTANCE: invention relates to the constructed recombinant plasmid DNA that can be used in biotechnology for preparing human insulin. Recombinant DNA based on plasmid pKK 223-3 and encoding preproinsulin is inserted between restriction sites...

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Bibliographic Details
Main Authors MARKIN S.S, DENISENKO V.S, SEMENOV M.P, NECHAEV V.N, BOBKOV JU.G, SIVOV I.G, UVAROV V.JU
Format Patent
LanguageEnglish
Published 20.07.1998
Edition6
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:FIELD: molecular biology, genetic engineering, medicine. SUBSTANCE: invention relates to the constructed recombinant plasmid DNA that can be used in biotechnology for preparing human insulin. Recombinant DNA based on plasmid pKK 223-3 and encoding preproinsulin is inserted between restriction sites Eco RI and Hind III. Triplet encoding methionine is replaced for triplet that encodes arginine. This ensures to obtain proinsulin by enzymatic cleavage. EFFECT: recombinant plasmid indicated above, improved method of insulin preparing. 2 dwg, 1 exc
Bibliography:Application Number: RU19970114220