METHOD OF QUANTITATIVELY DETERMINING ANGIOGENIN IN BIOLOGICAL SAMPLE

FIELD: analytical methods. SUBSTANCE: invention relates to estimation of protein substances in diary, meet industries, veterinary, and medicine engineering, in particular, to quantitatively determining angiogenin in milk raw material. The latter is defatted via centrifugation and subjected to chroma...

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Bibliographic Details
Main Authors SHALYGINA A.M, IONOVA I.I, KOMOLOVA G.S, ROGOV I.A, TIKHOMIROVA N.A, RUSTAM'JAN JU.L
Format Patent
LanguageEnglish
Published 27.04.1998
Edition6
Subjects
CHEMISTRY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PHYSICS
TESTING
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Summary:FIELD: analytical methods. SUBSTANCE: invention relates to estimation of protein substances in diary, meet industries, veterinary, and medicine engineering, in particular, to quantitatively determining angiogenin in milk raw material. The latter is defatted via centrifugation and subjected to chromatographic purification to remove excess of pancreatic ribonuclease on column filled with blue agarose sorbent. Milk material, before and after being passed through blue agarose column, is controlled for content of pancreatic ribonuclease. Angiogenin is quantitatively determined using combined sample in which pancreatic ribonuclease and angiogenin competitively interact with placental ribonuclease inhibitor. EFFECT: increased sensitivity. 1 tbla
Bibliography:Application Number: RU19960124515