PROCESS FOR PREPARING STABLE SUSPENSIONS OF SILICA AND SELENIUM NANOPARTICLES

• Main Authors: CĂLIN MARIANA, OANCEA FLORIN, CONSTANTINESCU-ARUXANDEI DIANA, RĂUT IULIANA
• Format: Patent
• Language: English; Romanian
• Published: 29.11.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; COMPOUNDS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FERTILISERS; FERTILISERS PRODUCING CARBON DIOXIDE; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; INORGANIC CHEMISTRY; INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; MANUFACTURE THEREOF; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NON-METALLIC ELEMENTS; ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention relates to a process for preparing stable suspensions of associates zerovalent selenium and silica nanoparticles, intended for application as plant biostimulants, to increase resistance to abiotic stress factors, to enhance plant nutrients uptake and use and to improve yield quality. Process according to the invention includes the following steps: cultivation of microbial strains highly producing amphiphile proteins with affinity for carbohydrates, on a mediums that promote the induction of those genes, separation of the plant biostimulants fungal mycelium and adding over biosilica rich plant material together with sterile minimal mineral medium, incubation, together with amphiphile proteins recovered from the original medium, removal of the mycelium and plant material, aseptic adding of a 10 mM sodium selenite solution, in Supernatant containing spores, excess amphiphile proteins, silica nanoparticles, incubation for the formation of the selenium nanoparticles, followed by tangential ultrafiltration separation of the newly formed mycelium, concentration of nanoparticles suspension resulted as permeate through tangential ultrafiltration, followed by its sterilization by ultrafiltration.