PROCESS FOR BIO-TESTING STRIGOLACTONE ANALOGUES

• Main Authors: CĂLIN MARIANA, OANCEA FLORIN, JECU MARIA-LUIZA, DONI MIHAELA, RĂUT IULIANA, ARSENE MELANIA LILIANA
• Format: Patent
• Language: English; Romanian
• Published: 30.04.2020
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention relates to a process for bio-testing strigolactone analogues in respect of their capacity of inducing/stimulating the production of volatile bioactive compounds in the microorganisms associated to crop plants. According to the invention, the process comprises the following stages: aseptic distribution of a hydro-jellified culture medium with a triblock copolymer and homogenization of strigolactone analogues in a plate with 24 wells, the inoculation of the agarized medium with microorganisms beneficial to the plants which produce volatile compounds and which positively respond to strigolactones, the plate covering and incubation for 48 h at 25°C, the placement of the plate wherein the isolates to be tested developed onto an identical plate with the biological system in respect of which the biologically-active volatile compounds are tested, the incubation of the joined plates for 2...7 days at 25°C, the determination of the effect upon the bio-testing system and the identification of the strigolactone analogues which produced the said effect.