SEPARATING DEVICE FOR MODIFYING CELL CULTURE PLATE, CELL CULTURE PLATE MODIFIED THEREBY AND METHOD FOR MEASURING CELL MOVEMENT, IN REAL TIME, IN IN-VITRO SYSTEM, USING CELLULAR IMPEDANCE

The invention relates to a device, a modified culture plate and a method using the same, for measuring, in real time, the cell movement in ex-vivo system. According to the invention, the device is a rectangular frame, made of polypropylene, having a width of 1 mm, a length of 4.9 mm, a height of 123...

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Bibliographic Details
Main Authors COROTCHI MARIA-CRISTINA, POPA MIREL ADRIAN
Format Patent
LanguageEnglish
Romanian
Published 29.06.2018
Subjects
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
SPIRITS
TESTING
VINEGAR
WINE
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Summary:The invention relates to a device, a modified culture plate and a method using the same, for measuring, in real time, the cell movement in ex-vivo system. According to the invention, the device is a rectangular frame, made of polypropylene, having a width of 1 mm, a length of 4.9 mm, a height of 123 mm and a contact surface of 4.9 mm. The claimed culture plate is a cell culture plate with wells having a surface of 0.2 cm, or a culture plate with golden microelectrodes with 96 or 16 wells, respectively, in which a detachable device, as claimed by the invention, is inserted, positioned along the well diameter, the device area of contact with the well bottom being insulated with 5 μl of biocompatible protein per well. The claimed method consists in cultivating 7000 adherent human cells per well, re-suspended in a volume of 100 μl of medium, after which the device is removed and cell migration is measured using an in vitro system, in real time, using the cellular impedance.
Bibliography:Application Number: RO20160000357