PURIFICATION PROCESS OF INSULIN

The process comprises dissolving the raw insulin, after removal of alcohol from cattle or pig pancreas processing, in hydroacetone buffer soln. of ammonium acetate (pH = 6.1). Pptn. in the presence of zinc ions, for dissolving at acid pH and reprecipitation of alkaline pH in the presence of NaCl, ED...

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Main Authors GHIOCEL RADU RAOUL, RO, BALEANU MIHAELA, RO, TIUTIU FLORISANDA, RO, CHIVU VIORICA, RO, MARINICA THEODOR, RO, BORZA VIRGINIA, RO, NICULESCU EUGENIA, RO
Format Patent
LanguageEnglish
Romanian
Published 25.11.1991
Edition4
Subjects
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
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Summary:The process comprises dissolving the raw insulin, after removal of alcohol from cattle or pig pancreas processing, in hydroacetone buffer soln. of ammonium acetate (pH = 6.1). Pptn. in the presence of zinc ions, for dissolving at acid pH and reprecipitation of alkaline pH in the presence of NaCl, EDTA and zinc ions, is followed by redissolving at acid pH and reprecipitation by addn. of NaCl. The insulin is crystallised to min. 24 UI/mg purity.
Bibliography:Application Number: RO19880136450