PROCESSO PARA A PREPARACAO DE POLIPEPTIDOS CONTENDO PROTEINA MACROFAGICA INFLAMATORIA HUMANA MIP-2 ALFA POR METODOS RECOMBINANTES E DE COMPOSICOES FARMACEUTICAS QUE OS CONTEM

• Main Authors: CAROL GALLEGOS, PATRICIA TEKAMP-OLSON
• Format: Patent
• Language: Portuguese
• Published: 30.11.1998
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TESTING; VINEGAR; WINE

An attempt to identify the human counterpart of murine macrophage inflammatory protein 2 unexpectedly resulted in not one but two candidate sequences, designated hu-MIP-2 alpha and hu-MIP- beta , respectively. By using the DNA sequences and recombinant systems provided by this invention, one can obtain hu-MIP-2 alpha , one of the two human counterparts to mMIP-2, substantially free of other human protein. This invention also provides antibodies reactive with hu-MIP-2 alpha polypeptides as well as immunoassay methods for determining hu-MIP-2 alpha and nucleotide probes for detecting nucleotide sequences encoding hu-MIP-2 alpha in biological samples.