PROCESSO DE FLOCULACAO - COAGULACAO PARA A OBTENCAO DE CALDOS DE FERMENTACAO DE STREPTOMYCES SP. CLARIFICADOS

It comprises: (a) adding an inorganic electrolyte to the broth; (b) incorporating an inorganic coagulant with stirring and at a pH of 5-8 in order to start the flocculation; (c) adding, once the flocculation has started, an organic polyelectrolyte at a pH of 6-8 and with stirring; (d) separating the...

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Bibliographic Details
Main Authors MARIA CONCEPCION GRACIA ALVAREZ, FERNANDO DE LA CALLE VERDU, GUILLERMO DIAZ DEL AGUILLA SARDA, MARIA PILAR J. DE URRIES SENANTE
Format Patent
LanguagePortuguese
Published 29.02.1996
Edition6
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:It comprises: (a) adding an inorganic electrolyte to the broth; (b) incorporating an inorganic coagulant with stirring and at a pH of 5-8 in order to start the flocculation; (c) adding, once the flocculation has started, an organic polyelectrolyte at a pH of 6-8 and with stirring; (d) separating the flocculi by means of rolling sieves or simple filtration, followed by decantation if the flocculation has been done in the presence of an organic solvent; and, optionally, concentrating the liquid by reverse osmosis or evaporation. The process is applicable in the clarification of culture broths allowing a micelle separation simultaneous to deproteinization. Likewise, the colloidal material or material in suspension is eliminated along with dissolved materials which make the purification process difficult.
Bibliography:Application Number: PT19900093314