NUCLEIC ACID PURIFICATION

A process for purifying plasmid DNA from a nucleic acid containing sample comprising plasmid DNA and contaminants, which process comprises a step of contaminant removal, comprising: (a) treating the sample to form a nucleic acid solution having a concentration of monovalent cations; (b) contacting t...

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Bibliographic Details
Main Authors RIMANTAS KVEDERAS, ALGIMANTAS MARKAUSKAS, ASTA SIKSNIUTE
Format Patent
LanguageEnglish
Portuguese
Published 23.12.2008
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A process for purifying plasmid DNA from a nucleic acid containing sample comprising plasmid DNA and contaminants, which process comprises a step of contaminant removal, comprising: (a) treating the sample to form a nucleic acid solution having a concentration of monovalent cations; (b) contacting the nucleic acid solution with an ultrafiltration membrane having a molecular weight exclusion limit of at least 30kDa under conditions in which substantially no gel-layer forms and in which the concentration of monovalent cations is sufficiently high for a time sufficient to remove substantially all RNA and form a retentate containing plasmid DNA; and (c) collecting the retentate.
Bibliography:Application Number: PT20020252249T