METHOD OF INACTIVATING THE GENES CODING PHENYL ACETATE CATABOLISING ENZYMES, PLASMIDES USES IN THAT METHOD AND STRAINS TRANSFIRMED BY MEANS OF SUCH PLASMIDES

Procedure for the inactivation of genes that code for phenylacetate catabolism enzymes, plasmids containing them, and strains transformed with them. The procedure is preferably applied to the phacA gene of A. nidulans and to the pahA gene of P. chrysogenum, genes which code respectively for enzymes...

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Main Authors MINGOT ASCENCAO JOSE MANUEL, SALTO MALDONADO FRANCISCO, DIEZ GARCIA BRUNO, VITALLER ALBA ALEJANDRO, RODRIGUEZ SAIZ MARTA, BARREDO FUENTE JOSE LUIS, PENALVA SOTO MIGUEL ANGEL, FERNANDEZ CANON JOSE MANUEL
Format Patent
LanguageEnglish
Published 24.05.1999
Edition6
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Procedure for the inactivation of genes that code for phenylacetate catabolism enzymes, plasmids containing them, and strains transformed with them. The procedure is preferably applied to the phacA gene of A. nidulans and to the pahA gene of P. chrysogenum, genes which code respectively for enzymes competing for this substrate with penicillin biosynthesis enzymes. The non-expression of these enzymes has a beneficial effect on the synthesis of this antibiotic, increasing its yield, with a lower phenylacetate demand per unit of culture.
Bibliography:Application Number: PL19980330464