IMPROVED ACYLTRANSFERASE POLYNUCLEOTIDES, POLYPEPTIDES, AND METHODS OF USE

• Main Authors: ROBERTS, NICHOLAS JOHN, WINICHAYAKUL, SOMRUTAI, SCOTT, RICHARD WILLIAM, CURRAN, AMY CHRISTINA, TAYLOR, DAVID CHARLES, MARILLIA, ELIZABETH-FRANCE, ROLDAN, MARISSA
• Format: Patent
• Language: English; Polish
• Published: 28.06.2019
• Subjects: ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE ORUNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FIRELIGHTERS; FODDER; FOODS OR FOODSTUFFS; FUELS; FUELS NOT OTHERWISE PROVIDED FOR; HUMAN NECESSITIES; LIQUEFIED PETROLEUM GAS; LUBRICANTS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NATURAL GAS; PEAT; PETROLEUM, GAS OR COKE INDUSTRIES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BYSUBCLASSES C10G, C10K; TECHNICAL GASES CONTAINING CARBON MONOXIDE; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

The invention provides chimeric DGAT1 proteins comprising: a) at their N-terminal ends, an N-terminal portion of a first DGAT1 protein, and b) at their C-terminal ends, a C-terminal portion of a second DGAT1 protein. The chimeric DGAT proteins show enhanced activity relative to at least one of the first DGAT1 protein and the second DGAT1 protein. The chimeric DGAT proteins of the invention can be expressed in cells to increase cellular lipid accumulation and/or modify the cellular lipid profile. The invention also provides polynucleotides encoding the chimeric DGAT1 proteins, cells and compositions comprising the polynucleotides or chimeric DGAT1 proteins, and methods using the chimeric DGAT1 proteins to produce oil.