VARIANT ALPHA-AMYLASES FROM BACILLUS SUBTILIS AND METHODS OF USE, THEREOF

Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars...

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Main Authors PEPSIN, MICHAEL J, TOPPOZADA, AMR R, WALLACE, LOUISE, LEE, SANG-KYU, SHAW, ANDREW, POWER, SCOTT D, CUEVAS, WILLIAM A, REQUADT, CAROL A, RAMER, SANDRA W, CHIN, REGINA, ESTELL, DAVID A
Format Patent
LanguageEnglish
Polish
Published 31.08.2015
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
Bibliography:Application Number: PL20090759577T