ACILTRANSFERASA Y GEN QUE CODIFICA ACILTRANSFERASA

• Main Authors: STEFFENS, JOHN C, GHANGAS, GURDEV S
• Format: Patent
• Language: Spanish
• Published: 25.12.1998
• Subjects: ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS,e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES,DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; CONVEYING; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HANDLING THIN OR FILAMENTARY MATERIAL; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PACKAGES; PACKAGING ELEMENTS; PACKING; PERFORMING OPERATIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; STORING; TRANSPORTING; VINEGAR; WINE

SE REFIERE A UNA ENZIMA ACILTRANSFERASA PURIFICADA QUE COMPRENDE: LA SECUENCIA PEPTIDICA (8) Y SIETE FRACCIONES DE PEPTIDOS OBTENIDOS POR HIDROLISIS DE LA SECUENCIA, DICHA ACILTRANSFERASA CATALIZA LA TRANSFERENCIA ANOMERICA DEL SUSTITUYENTE ACILADO DE 1-O-ACIL-�-D-GLUCOSA A GLUCOSA, 2-DESOXIGLUCOSA, 3-O-METILGLUCOSA O GLUCOSA PARCIALMENTE ACILADA Y CATALIZA LA DESPROPORCION ENTRE DOS MOLECULAS DE 1-O-ACILO-�-D-GLUCOSA QUE RESULTAN EN LA FORMACION DE DIACILGLUCOSA Y GLUCOSA. TAMBIEN SE REFIERE AL GEN QUE CODIFICA LA ACILTRANSFERASA, GEN QUE SE OBTUVO DEL GENOMA DE UNA ESPECIE DE TOMATE SILVESTRE LYCOPERSICON PENNELLI (LA716), CUYA SEMILLA ORIGINAL FUE OBTENIDA DE LOS ANDES PERUANOS DEL SUR. ADEMAS SE REFIERE AL PROCEDIMIENTO DE PREPARACION DE LOS ESTERES DE PALMITOILO DE GLUCOSA QUE COMPRENDE REACCIONAR 1-O-PALMITOIL-�-GLUCOSA CON GLUCOSA O ESTERES PARCIALES DE PALMITOILO DE GLUCOSA, EN PRESENCIA DE UNA ACILTRANSFERASA DE SECUENCIA (8), DICHO PROCEDIMIENTO QUE NO EXISTE EN LA NATURALEZA, PERMITE LA ACILACION PARCIAL DE LOS HIDROXILOS DISPONIBLES EN EL AZUCAR, OBTENIENDOSE POLIESTERES DE AZUCAR QUE SON UTILES COMO INSECTICIDAS, EMULSIONANTES O EDULCORANTES, ENTRE OTROS Essentially pure acyltransferase is provided which is functional to catalyze reaction to form sugar esters. Also provided is isolated gene encoding acyltransferase. Additionally provided is method for forming palmityl esters of glucose comprising reacting 1-O-palmitoyl- beta -D-glucose with itself, with glucose or with palmityl partial ester of glucose in the presence of a catalytically effective amount of acyltransferase.