Methods for transduction and cell processing

• Main Authors: Crisman, Ryan, Wood, Travis, Ramsborg, Chris
• Format: Patent
• Language: English
• Published: 30.06.2023
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL ORCHEMICAL PROCESSES; CENTRIFUGES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRANSPORTING; VINEGAR; WINE

Provided are methods, systems, and kits for cell processing, e.g., for therapeutic use, such as for adoptive cell therapy. The claimed method is for the stimulation of cells where the stimulation agent binds to a molecule expressed by a plurality of the primary cells. The incubation is carried out for a period of time from 8 to 30 hours where a portion of the incubation occurs in an internal cavity of a centrifugal chamber under mixing conditions from 5 minutes to 6 hours, and the mixing conditions are carried out at a speed from about 600 rpm to 1700 rpm. The incubation in some embodiments is carried out in an internal cavity of a generally rigid centrifugal chamber, such as a cylindrical chamber made of hard plastic, the cavity of which may have a variable volume. The methods include other processing steps, including those carried out in such a chamber, including washing, selection, isolation, culture, and formulation. In particular, the disclosure relates to method providing advantages over available processing methods, such as available methods for large-scale processing. Such advantages include, for example, reduced cost, streamlining, increased efficacy, increased safety, and increased reproducibility among different subjects and conditions.