Method for purifying antibody having low isoelectric point

The inventors of the present invention found that by using a protein A column to purify a low pI antibody, performing an acid treatment and a neutralisation treatment, and thereafter, when a fixed time period has elapsed, performing a step for eliminating antibody aggregates that have been produced,...

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Bibliographic Details
Main Authors Ueda, Yasufumi, Yanagita, Satoko, Kobayashi, Shohei, Fukunaga, Masahiro, Kawase, Takuo
Format Patent
LanguageEnglish
Published 28.10.2022
Subjects
CHEMISTRY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
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Summary:The inventors of the present invention found that by using a protein A column to purify a low pI antibody, performing an acid treatment and a neutralisation treatment, and thereafter, when a fixed time period has elapsed, performing a step for eliminating antibody aggregates that have been produced, subsequent antibody aggregation can be suppressed. Furthermore, the inventors found that by purifying a low pI antibody in the bind/elute mode of an anion exchange resin and further using a hydrophobic interaction chromatography or multimodal chromatography resin, it is possible to eliminate impurities more efficiently than conventional methods.
Bibliography:Application Number: NZ20140722057