Fermentive production of four carbon alcohols
A method for the recovery of isobutanol comprising culturing a recombinant microbial host cell in a fermentation medium under conditions whereby isobutanol is produced, and recovering the isobutanol; wherein said fermentation medium comprises a fermentable carbon substrate; and wherein said recombin...
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Main Authors | , , , , |
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Format | Patent |
Language | English |
Published |
29.05.2015
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Subjects | |
Online Access | Get full text |
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Summary: | A method for the recovery of isobutanol comprising culturing a recombinant microbial host cell in a fermentation medium under conditions whereby isobutanol is produced, and recovering the isobutanol; wherein said fermentation medium comprises a fermentable carbon substrate; and wherein said recombinant microbial host cell comprises a heterologous DNA molecule encoding polypeptides that catalyze substrate to product conversions for: i) pyruvate to acetolactate (pathway step a) ii) acetolactate to 2,3-dihydroxyisovalerate (pathway step b) iii) 2,3-dihydroxyisovalerate to α-ketoisovalerate (pathway step c) iv) α-ketoisovalerate to isobutyraldehyde, (pathway step d) and v) isobutyraldehyde to isobutanol, (pathway step e) wherein the DNA molecule is heterologous to said microbial host cell. |
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Bibliography: | Application Number: NZ20060610893 |