Fermentive production of four carbon alcohols

A method for the recovery of isobutanol comprising culturing a recombinant microbial host cell in a fermentation medium under conditions whereby isobutanol is produced, and recovering the isobutanol; wherein said fermentation medium comprises a fermentable carbon substrate; and wherein said recombin...

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Bibliographic Details
Main Authors DONALDSON GAIL K, ELIOT ANDREW C, FLINT DENNIS, MAGGIO-HALL LORI ANN, NAGARAJAN VASANTHA
Format Patent
LanguageEnglish
Published 29.05.2015
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A method for the recovery of isobutanol comprising culturing a recombinant microbial host cell in a fermentation medium under conditions whereby isobutanol is produced, and recovering the isobutanol; wherein said fermentation medium comprises a fermentable carbon substrate; and wherein said recombinant microbial host cell comprises a heterologous DNA molecule encoding polypeptides that catalyze substrate to product conversions for: i) pyruvate to acetolactate (pathway step a) ii) acetolactate to 2,3-dihydroxyisovalerate (pathway step b) iii) 2,3-dihydroxyisovalerate to α-ketoisovalerate (pathway step c) iv) α-ketoisovalerate to isobutyraldehyde, (pathway step d) and v) isobutyraldehyde to isobutanol, (pathway step e) wherein the DNA molecule is heterologous to said microbial host cell.
Bibliography:Application Number: NZ20060610893