Modifying glycoprotein production in plants

• Main Authors: THOMAS, PACCALET, PATRICE, LEROUGE, VERONIQUE, GOMORD, MARC-ANDRE, D'AOUST, LOIC, FAYE, LOUIS-PHILIPPE, VEZINA, STEPHANIE, AQUIN, CHRISTOPHE, RIHOUEY, MURIEL, BARDOR, CAROLE, BUREL, ESTELLE, MARQUET-BLOUIN, CHRISTOPHE, SOURROUILLE
• Format: Patent
• Language: English
• Published: 28.06.2013
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

598417 Modifying glycoprotein production in plants Provided is a method for synthesizing a protein of interest with reduced fucosylation and xylosylation comprising, introducing within a plant, a portion of a plant, or a plant cell, a nucleotide sequence encoding a first nucleotide sequence encoding beta-1,4-galactosyltransferase (GalT), the first nucleotide sequence operatively linked with a first regulatory region that is active in the plant, and a second nucleotide sequence for encoding the protein of interest, the second nucleotide sequence operatively linked with a second regulatory region that is active in the plant, and transiently co-expressing the first and second nucleotide sequences to synthesize the protein of interest comprising reduced fucosylation and xylosylation.