Methods and compositions for improving sugar transport, mixed sugar fermentation, and production of biofuels

• Main Authors: DU JING, SUN JIANPING, GALAZKA JONATHAN M, GLASS N. LOUISE, ZHAO HUIMIN, HA SUK JIN, TIAN CHAOGUANG, LI SIJIN, BEESON WILLIAM T. IV, KIM SOO RIN, CHOI JIN HO, YANG XIAOMIN, DOUDNA CATE JAMES H, JIN YONG-SU
• Format: Patent
• Language: English
• Published: 30.05.2014
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGYGENERATION, TRANSMISSION OR DISTRIBUTION; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; VINEGAR; WINE

Disclosed is a method of increasing transport of cellodextrin into a cell, comprising: -providing a non-human host cell, wherein the host cell comprises a recombinant polynucleotide encoding a polypeptide having the structure of a Major Facilitator Superfamily sugar transporter and comprising transmembrane αlpha-helix 1, αlpha-helix 2, αlpha-helix 3, αlpha-helix 4, αlpha-helix 5, αlpha-helix 6, αlpha-helix 7, αlpha-helix 8, αlpha-helix 9, αlpha-helix 10, αlpha-helix 11, αlpha-helix 12, and transmembrane αlpha-helix 1 comprises: -a leucine, isoleucine, valine or methionine at the position corresponding to amino acid 1 of SEQ ID NO: 1 -a tyrosine at the position corresponding to amino acid 2 of SEQ ID NO: 1; - a phenylalanine or leucine at the position corresponding to amino acid 3 of SEQ ID NO: 1; - a tyrosine or phenylalanine at the position corresponding to amino acid 17 of SEQ ID NO: 1; - an aspartate at the position corresponding to amino acid 18 of SEQ ID NO: 1; and -culturing the cell in a medium such that the recombinant polynucleotide is expressed, wherein expression of the recombinant polynucleotide results in increased transport of cellodextrin into the cell compared with a cell that does not comprise the recombinant polynucleotide. Note: The sequence identities are as disclosed in the specification.