FERMENTIVE PRODUCTION OF FOUR CARBON ALCOHOLS

• Main Author: DONALDSON, GAIL, K
• Format: Patent
• Language: English
• Published: 25.01.2013
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Provided is a recombinant microbial host cell comprising heterologous DNA molecules encoding polypeptides that catalyzes substrate to product conversions for i) pyruvate to acetolactate, (pathway step a) ii) acetolactate to 2,3-dihydroxyisovalerate, (pathway step b) iii) 2,3-dihydroxyisovalerate to alpha-ketoisovalerate, (pathway step c) iv) alpha-ketoisovalerate to valine, (pathway step h) v) valine to isobutylamine, (pathway step i) and vi) isobutylamine to isobutyraldehyde, (pathway step j); wherein the polypeptide that catalyzes a substrate to product conversion of pyruvate to acetolactate is acetolactate synthase, the polypeptide that catalyzes a substrate to product conversion of acetolactate to 2,3-dihydroxyisovalerate is acetohydroxy acid isomeroreductase, the polypeptide that catalyzes a substrate to product conversion of 2,3-dihydroxyisovalerate to alpha-ketoisovalerate is acetohydroxy acid dehydratase, the polypeptide that catalyzes a substrate to product conversion of alpha-ketoisovalerate to valine is transaminase, the polypeptide that catalyzes a substrate to product conversion of alpha-ketoisovalerate to valine is valine dehydrogenase, the polypeptide that catalyzes a substrate to product conversion of valine to isobutylamine is valine decarboxylase, and the polypeptide that catalyzes a substrate to product conversion of isobutylamine to isobutyraldehyde is omega transaminase; and wherein said microbial host cell produces isobutanol. The microbe can be a bacterium, cyanobacterium, a filamentous fungus or a yeast.