Method for modulating expression of (1,3;1,4)-beta-D-glucan synthases in cereal crops like barley encoded by the CslH gene family

• Main Authors: BURTON, RACHEL, ANITA, PETTOLINO, FILOMENA, ANGELA, BACIC, ANTONY, FINCHER, GEOFFREY, BRUCE, JOBLING, STEPHEN, ALAN, DOBLIN, MONIKA, SUSANNE
• Format: Patent
• Language: English
• Published: 21.12.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

Discloses a method for modulating the level of (1,3;1,4)-ß-D-glucan produced by a nonhuman cell, the method comprising modulating the level of expression and/or activity of a CsIH-encoded (1,3;1,4)-ß-D-glucan synthase in the cell. Discloses transgenic plants (e.g. cereal crop plants) comprising cells modified by the method disclosed. An isolated CsIH nucleic acid or a fragment thereof which is at least 94 % identical to SEQ ID NO:1 is disclosed. Further disclosed is an antibody or epitope binding fragment raised against the protein encoded by SEQ ID NO: 1.