PRODUCTION OF HIGH TRYPTOPHAN MAIZE BY CHLOROPLAST TARGETED EXPRESSION OF ANTHRANILATE SYNTHASE

• Main Authors: VARAGONA, MARGUERITE, J, NAVARRO, SANTIAGO, XAVIER, YE, GUANGNING, SHI, XIAOHONG, RAPP, WILLIAM, D, MANJUNATH, SIVA, WINSON, JENNIFER, L
• Format: Patent
• Language: English
• Published: 29.04.2011
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; TRAPPING; VINEGAR; WINE

An expression vector for increasing tryptophan production in a plant. The vector comprises a polynucleotide molecule encoding a chloroplast transit peptide (CTP) fused to a monomeric anthranilate synthase, wherein; (a) the CTP comprises the polypeptide sequence of SEQ ID NO: 21, 22, 28, 32, 33 or 36; or (b) the polynucleotide molecule encoding the CTP comprises SEQ ID NO: 2, 3, 9, 13, 14 or 17. The CTP provides enhanced targeting of the monomeric anthranilate synthase to the chloroplast of the plant cell when compared with the targeting of the anthranilate synthase by chloroplast transit peptide 1 (CTP1).