Method of culturing thermophilic microorganisms suitable for the production of ethanol

• Main Authors: CRIPPS, ROGER, ELEY, KIRSTIN, ATKINSON, ANTHONY
• Format: Patent
• Language: English
• Published: 30.07.2010
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGYGENERATION, TRANSMISSION OR DISTRIBUTION; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; VINEGAR; WINE

Disclosed is a method for the production of thermophilic microorganisms suitable for the production of ethanol, comprising: i. culturing a thermophilic microorganism under aerobic or anaerobic conditions in a suitable culture media; and ii. incorporating amounts of ethanol into the culture media to induce ethanol tolerance; wherein the wild-type thermophilic microorganism is modified by inactivation of the native lactate dehydrogenase gene, and wherein the native lactate dehydrogenase gene, or a portion, thereof, has been deleted.