Analyzing a sample of biomolecular analyte using electrophoresis

Disclosed is ruggedized separation device (10) for analyzing a sample of bimolecular analyte using electrophoresis. The device includes: a test module (55); a holder (20) configured to support test module; an electrophoresis device (30) connected to the holder; a light source; a light detector; a pl...

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Bibliographic Details
Main Authors LAM, HEUNG CHUAN, KELLOGG, GREG, TAN, EUGENE
Format Patent
LanguageEnglish
Published 31.03.2011
Subjects
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
CHEMISTRY
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING ELECTRIC VARIABLES
MEASURING MAGNETIC VARIABLES
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PHYSICS
SPIRITS
TESTING
TRANSPORTING
VINEGAR
WINE
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Summary:Disclosed is ruggedized separation device (10) for analyzing a sample of bimolecular analyte using electrophoresis. The device includes: a test module (55); a holder (20) configured to support test module; an electrophoresis device (30) connected to the holder; a light source; a light detector; a plurality of optical devices; and a base plate onto which the light source, the light detector, and the plurality of optical devices are secured. The test module comprises one microfluidic channel disposed within a member formed of a transparent material, the microfluidic channel inelectrical communication with an anode (100) and a cathode (102). The microfluidic channel comprises: a separation portion; a waste arm in fluid communication with a waste arm opening, the waste arm opening is in fluid communication with a single separation portion; and a sample arm in fluid communication with a sample arm opening. The electrophoresis device comprises the anode and the cathode, and applies one or more biasing configurations to the test module. The light source emits a light beam that is transmitted to the test module and excites fluorescence in the sample of biomolecular analyte, the fluorescence being detected by the light detector. The optical devices transmit the light beam to the test module and the fluorescence from the test module to the light detector.
Bibliography:Application Number: NZ20060563831