Mammary gland tissue-specific expression system using beta-casein promoter site of Korean native goat

Disclosed are mammary gland tissue-specific expression systems using the promoter site for the beta -casein gene of Korean native goats. Physiological activating substances can be produced by use of these expression systems. In each of the expression systems, that is, novel plasmids pGbc, pGbc_L and...

Full description

Saved in:
Bibliographic Details
Main Authors HAN, YOUNG MAHN, LEE, KYUNG KWANG, KIM, SUN JUNG, OH, WON JUN, KO, JUNG HO, JEONG, HAE YOUNG, YOO, OOK JOON
Format Patent
LanguageEnglish
Published 26.04.2002
Edition7
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CARE OF BIRDS, FISHES, INSECTS
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW BREEDS OF ANIMALS
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR
SPIRITS
TRAPPING
VINEGAR
WINE
Online AccessGet full text

Cover

More Information
Summary:Disclosed are mammary gland tissue-specific expression systems using the promoter site for the beta -casein gene of Korean native goats. Physiological activating substances can be produced by use of these expression systems. In each of the expression systems, that is, novel plasmids pGbc, pGbc_L and pGbc_S (deposition Nos. KCTC 0515BP, 0514BP and 0513BP, respectively), a beta -casein gene expression-regulating region, a physiological activating substance gene and a termination-regulating region are linked. Human granulocyte colony stimulating factor (hG-CSF) or human granulocyte macrophage colony stimulating factor (hGM-CSF) can be produced in HC11 cells, a mouse mammary gland tissue-derived cell line, and in the milk secreted from the transgenic mice by use of a hG-CSF or hGM-CSF gene-carrying pGbc, pGbc_L or pGbc_S in transfection into cell and microinjection to mouse. The proteins experience posttranslational modification and maintain their normal activity in the human body. The expression systems make it possible to produce the proteins in large amounts, to scale up protein production to the extent of industrialization, and to isolate and purify the desired protein.
Bibliography:Application Number: NZ19980505218