PREPARATION OF ENZYMES, MODIFICATIONS, CATALYTIC TRIADS TO ALTER RATIOS OR TRANSESTERIFICATION/HYDROLYSIS RATIOS
The invention relates to altering the transesterification rate/hydrolysis rate ratio and nucleophile specificity in reactions that are catalyzed by enzymes with catalytic triads such as serine hydrolases and cystine hydrolases by using a novel method to modify the enzyme. Modifications to the enzyme...
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Main Authors | , , , |
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Format | Patent |
Language | English |
Published |
28.04.1993
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Edition | 5 |
Subjects | |
Online Access | Get full text |
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Summary: | The invention relates to altering the transesterification rate/hydrolysis rate ratio and nucleophile specificity in reactions that are catalyzed by enzymes with catalytic triads such as serine hydrolases and cystine hydrolases by using a novel method to modify the enzyme. Modifications to the enzyme are achieved by altering amino acid residues within 15 angstroms of the catalytic triad in the enzyme. |
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Bibliography: | Application Number: NZ19870221627 |