Method for Determining Purity of Nicotine by Use of GCMS-GC Method

• Main Authors: WEI LIU, KE ZHANG, HAOWEI SUN, ZILONG ZHANG, XUE JIANG, JIANLONG GU, XIAOWEI ZHANG, WEILI KOU, HAIYAN LI, SHIHAN LIANG, JIEYUN CAI, CHUNQIONG WANG, JIE LONG, CHAO LI, DAN LI
• Format: Patent
• Language: English
• Published: 31.03.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The present invention discloses a method for determining a purity of nicotine by use of a GCMS-GC (gas chromatography mass spectrometry-gas chromatography) method, including: Step A: preliminarily determining a purity (nic%) of the nicotine by use of a GC/MS method; Step B: determining a moisture content (wat%) of the nicotine by use of a GC method; and Step C: carrying out correction to obtain an exact purity (nic%) of the nicotine, wherein a correction formula is expressed as NIC% = nic%*(100% - wat%). The present invention establishes a method for determining a purity of nicotine by use of a gas chromatography mass spectrometry combined with a gas chromatography. Compared with a gravimetric method using a tungstosilicic acid widely used at present, because in this method, after a modern chromatographic technique is utilized to efficiently separate a sample, the molecular weight (one of inherent physicochemical constants) of nicotine is taken as a detection target, the method is high in specificity for nicotine detection and uneasy to be interfered by other impurities, especially alkaline impurities (alkaloids, inorganic bases) in the sample, and accordingly, when being provided for carrying out purity detection on nicotine samples, especially those samples laid long and with high impurity contents, the method is easy to operate, has a strong separation capacity and produces reliable qualitative and quantitative results. Thus, the method may be promoted as a rapid detection method for nicotine purity.