PRIMER PROBE SET AND KIT FOR RT-PCR DETECTION OF HUMAN LEUKOTRIENE RECEPTOR CYSLTR2 MRNA

• Main Authors: SHANDONG WU, XUEHAN JIANG, LEI QIAN, SHIDAN LI, YI LIU, XUKAI YANG, ZHOUJIE WU
• Format: Patent
• Language: English
• Published: 21.02.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

L The present disclosure relates to a primer probe set and a kit for reverse transcription-polymerase chain reaction (RT-PCR) detection of a human leukotriene receptor CYSLTR2 mRNA, belonging to the technical field of biological detection. In the present disclosure, the primer probe set includes a primer CysLTRZ-F, a primer CysLTRZ-R and a probe CZ-Probe; where the primer CysLTRZ-F has a nucleotide sequence shown in SEQ ID NO. l, the primer CysLTRZ-R has a nucleotide sequence shown in SEQ ID NO.2, and the probe CZ-Probe has a nucleotide sequence shown in SEQ ID No.3. The primer probe set of TagMan real-time fluorescence quantitative one-step RT-PCR detection is established for a human CysLTRZ, and the primer probe set provides a detection method with high accuracy, wide detection range and high sensitivity for the CysLTRZ protein.