METHOD FOR INCREASING RESISTANCE AND YIELD OF RESISTANT DEXTRIN

• Main Authors: YANJUN TIAN, HUI XU, YANHONG HUANG, LIPING YANG, LIPING LIU, JUNJIAO ZHANG, JIANJUN LIU
• Format: Patent
• Language: English
• Published: 17.08.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC MACROMOLECULAR COMPOUNDS; POLYSACCHARIDES; SPIRITS; THEIR PREPARATION OR CHEMICAL WORKING-UP; VINEGAR; WINE

The disclosure provides a method for increasing resistance and yield of resistant dextrin, belonging to the preparation field of water-soluble dietary fibers. The method of the disclosure comprises: isoamylase enzymolysis, d-amylase enzymolysis and transglucosidase enzymolysis is successively carried out on pyrodextrin, and after enzymolysis is completed, ethanol is added into enzymatic hydrolysate for alcohol precipitation so as to obtain resistant dextrin. According to the disclosure, through addition of isoamylase enzymolysis step, small branched chains in pyrodextrin become a plurality of small straight chains, on the one hand, d-amylase can more thoroughly disconnect (uc-1,4 glucosidic bond in pyrodextrin to increase the resistance of resistant dextrin, the content of small-molecular glucose or maltose or the like in the enzymatic hydrolysate is increased to a certain extent and then more small-molecular glucose and maltose are provided for transglucosidase, the content of (uc-1,6 glucosidic bond is further increased, and the resistance of resistant dextrin is promoted. The method of the disclosure is convenient to operate and low in cost. Furthermore, the obtained resistant dextrin product is good in resistance, high in yield and suitable for industrial application.