CHIMERIC CHAINS THAT CODE FOR PROTEINS THAT INDUCE EFFECTS DIRECTED AGAINST VIRUSES

• Main Author: IRIS VALDES PRADO
• Format: Patent
• Language: English; Spanish
• Published: 07.03.2005
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention relates to the production of chimeric chains that code for proteins that induce in the receptor effects against viruses. Said proteins stimulate the production of a specific serotype humoral response and protection against infection by Dengue viruses, thereby preventing the effects of non-specific serotype viral amplification which cause haemorrhages and clinical complications which are associated with this type of pathology. The nucleic acid chains comprise a specific combination of fragments belonging to the gene of a mutant protein having N.meningitidis dehydrogenase activity and fragments that code for a region of protein E of the envelope of the Dengue viruses. Moreover, when inserted in an expression vector, said nucleic acid chains give rise to chimeric proteins having specific characteristics. The chimeric molecules resulting from said invention can be used in the pharmaceutical industry to produce highly-serotype-specific diagnoses and vaccine preparations intended for humans. Obtencion de cadenas quimericas codificantes para proteinas que inducen en el receptor efectos contra virus, las cuales estimulan la produccion de una respuesta humoral serotipo especifica y proteccion frente a la infeccion por los virus del Dengue, evitandose los efectos de amplificacion viral serotipo inespecificos causantes de las hemorragias y complicaciones clinicas descritas en este tipo de patologia. Estas cadenas de acidos nucleicos estan compuestas por la combinacion especifica de fragmentos pertenecientes al gen de una proteina mutante con actividad deshidrogenasa de N.meningitidis y fragmentos que codifican para una region de la proteina E de la envoltura de los virus del Dengue, las cuales al insertarse en un vector de expresion, dan como resultado las proteinas quimericas con caracteristicas especificas. Las moleculas quimericas resultantes de esta invencion son aplicables a la industria farmaceutica en la obtencion de preparados vacunales y diagnosticos de alta especificidad serotipica para uso en humanos.