VR-2332 VIRAL NUCLEOTIDE SEQUENCE AND METHODS OF USE

• Main Authors: MARGARET R. ELAM, LAURA T. KAKACH, MICHAEL P. MURTAUGH
• Format: Patent
• Language: English; Spanish
• Published: 31.05.1997
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; SUGARS; TESTING; VINEGAR; WINE

A nucleotide sequence is provided for the VR-2332 virus, which is capable of causing Porcine Reproductive and Respiratory Syndrome. The nucleotide sequence includes protein coding regions that are inserted into recombinant vectors for the host expression of viral proteins according to a variety of vaccination techniques. Diagnostic assays utilize fragmentary sequences or oligonucleotides to selectively identify the VR-2332 nucleic acids by hybridization or PCR amplification reactions that distinguish VR-2332 nucleotide sequences from other PRRS-causative viruses which are immunologically distinct from VR-2332. Se proporciona una secuencia viral de nucleotidos del virus VR-2332, la cual es capaz de producir el síndrome reproductivo y respiratorio porcino. La secuencia de nucleotidos incluye regiones que codifican para proteína las cuales se insertan en vectores recombinantes de la expresion en huésped de proteínas virales de acuerdo con una variedad de técnicas de vacunacion. Los ensayos diagnosticos utilizan secuencias fragmentarias de oligonucleotidos para identificar selectivamente los ácidos nucleicos de VR-2332 mediante hibridizacion o reacciones de amplificacion por PCR que diferencían a las secuencias de nucleotido de VR-2332 de otros virus causantes de PRRS, los cuales son inmunologicamente diferentes de VR-2332.