LOWERING SATURATED FATTY ACID CONTENT OF PLANT SEEDS

• Main Authors: Ann Owens MERLO, Mark A. THOMPSON, Terence A. WALSH, Scott BEVAN, Daniel J. GACHOTTE
• Format: Patent
• Language: English; Spanish
• Published: 07.09.2017
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

Compositions and methods include genetically encoding and expressing a novel delta-9 desaturase in plant cells. In some embodiments, methods of expressing nucleic acids in a plant cell to take advantage of the delta-9 desaturase enzyme's activity, such that the percent composition of saturated fatty acids in plant seeds is decreased and there is a concomitant increase in ω-7 fatty acids. In other embodiments, amino acid sequences have delta-9 desaturase activity. Methods can involve expression of delta-9 desaturase in plant cells, plant materials, and whole plants for the purpose of increasing the amount of unusual fatty acids in whole plants, plant seeds, and plant materials, for example, seeds. La presente invención se refiere a las composiciones y métodos incluyen codificar y expresar genéticamente una desaturasa delta-9 novedosa en células de planta. En algunas modalidades, los métodos para expresar ácidos nucleicos en una célula de la planta, toman la ventaja de la actividad de la enzima de desaturasa delta-9, de modo que se disminuya el porcentaje en la composición de los ácidos grasos saturados en las semillas de la planta y exista un incremento concomitante en los ácidos grasos ?-7. En otras modalidades, las secuencias de aminoácido tienen actividad de desaturasa delta-9. Los métodos pueden implicar la expresión de la desaturasa delta-9 en células de planta, materiales de planta y plantas completas, con el propósito de incrementar la cantidad de ácidos grasos inusuales en plantas completas, semillas de planta y materiales de la planta, por ejemplo, semillas.